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Reliable Wastewater Surveillance Starts with an Efficient Extraction Workflow

Recovering high-quality nucleic acids from wastewater remains one of the biggest challenges in wastewater-based epidemiology (WBE). Complex sample matrices, low pathogen concentrations, and PCR inhibitors all demand an extraction workflow that is both efficient and consistent.

Tianlong's wastewater pathogen surveillance solution combines the GeneRotex 24 Automated Nucleic Acid Extraction System with the T851H WastewaterPathogenDNAandRNAExtraction Kits, providing a streamlined workflow for reliable nucleic acid recovery from wastewater samples.

· Fast turnaround with minimal sample input
The complete enrichment and extraction workflow can be finished in approximately 45 minutes using just 10 mL of wastewater, enabling faster laboratory throughput while reducing sample volume requirements.

Proven performance with real wastewater samples

PMMoV (Pepper Mild Mottle Virus) is widely recognized as a process control marker in wastewater surveillance due to its abundance and stability in human fecal waste. Higher PMMoV recovery generally reflects more efficient nucleic acid recovery and a more robust extraction process.

In the evaluation of 62 real wastewater samples, Tianlong's solution achieved an average PMMoV recovery rate of approximately 50%, demonstrating reliable nucleic acid enrichment from complex wastewater matrices.

The workflow also delivered excellent precision for both PMMoV and SARS-CoV-2 detection, ensuring high reproducibility and consistent molecular testing results.

Validated across multiple public health laboratories

The solution has been evaluated through multicenter studies conducted by several CDC laboratories. Compared with PEG precipitation, ultrafiltration, and multiple commercially available extraction kits, Tianlong's workflow demonstrated:

· Higher nucleic acid recovery efficiency
· Improved analytical sensitivity
· Earlier Ct values for SARS-CoV-2 detection
· Consistent performance across laboratories and testing conditions

Designed for public health laboratories, environmental surveillance programs, and large-scale monitoring initiatives, Tianlong's wastewater extraction solution helps simplify laboratory workflows while delivering reliable molecular results for wastewater pathogen surveillance.