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  ["title"] => string(72) "How to Reduce Chances of Lab Contamination During DNA and RNA Extraction"
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  ["content"] => string(11472) "<p>In pursuing molecular studies, researchers frequently encounter the formidable challenge of lab contamination, particularly during <a href="https://www.medtl.net/products/instruments/nucleic-acid-extractors" target="_self">DNA and RNA extraction</a> processes. Such contamination not only compromises the integrity of scientific data but can lead to significant delays and increased costs in research.</p><p>As scientists seek to unlock the secrets held within genetic materials, extraneous nucleic acids can severely skew results, leading to misleading conclusions. Given the critical importance of accurate data in molecular biology, addressing lab contamination is paramount.</p><p>This article delves into practical strategies to mitigate contamination risks during the crucial stages of DNA and RNA extraction, ensuring more reliable and accurate research outcomes.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780211312033.png" title="" alt="1.png" width="807" height="533" style="width: 807px; height: auto;"/></p><p><strong>DNA and RNA Extraction Processes: Manual and Automated Methods</strong></p><ul style="list-style-type: disc;"><li><p>Manual Steps</p></li></ul><p>The process of manually extracting DNA and RNA begins with cell lysis, where cell membranes are disrupted to release nucleic acids. This is often achieved through physical means like grinding or using chemical lysing agents. Subsequent steps involve treating the solution to remove proteins and other contaminants, typically using enzymes such as protease or organic solvents.</p><p>After purification, nucleic acids are precipitated from the solution, usually with alcohol, and collected by centrifugation. The final step is washing and resuspending the DNA or RNA in a suitable buffer, preparing it for further analysis.</p><ul style="list-style-type: disc;"><li><p>Automatic Solution: Automated Nucleic Acid Extraction Systems</p></li></ul><p>Automated nucleic acid extraction systems provide a more streamlined and standardized approach compared to manual methods. These systems utilize robotic mechanisms and pre-packed cartridges containing all necessary reagents, processing multiple samples simultaneously.</p><p>The automated process follows the core steps of lysis, purification, and recovery, integrated into a single, closed system. This not only improves efficiency but also minimizes the risk of contamination and human error. Automated systems are particularly valuable in high-throughput settings, enhancing both reproducibility and scalability.</p><p><strong>Risks of Lab Contamination During DNA and RNA Extraction</strong></p><p>Contamination during the extraction of DNA and RNA can occur at various stages, primarily due to the sensitive nature of the procedures involved. Understanding these risks is vital&nbsp;for implementing effective countermeasures:</p><ul style="list-style-type: disc;"><li><p>Cross-Contamination</p></li></ul><p>Handling multiple samples can simultaneously lead to cross-contamination if equipment and surfaces are not properly sanitized between procedures.</p><ul style="list-style-type: disc;"><li><p>Environmental Contaminants</p></li></ul><p>Airborne particles, microbial contamination, and even previously amplified DNA can introduce nucleic acid contaminants into the sample.</p><ul style="list-style-type: disc;"><li><p>Reagent Contamination</p></li></ul><p>Reagents used in extraction processes can be contaminated with DNA or RNA from their manufacturing environment, potentially leading to false results.</p><ul style="list-style-type: disc;"><li><p>Human Error</p></li></ul><p>Mishandling of samples, improper use of equipment, and failure to follow protocols meticulously can all introduce contaminants into the extraction process.</p><p><strong>Strategies to Minimize Lab Contamination</strong></p><p>To safeguard the integrity of DNA and RNA extraction processes, several proactive strategies can be employed:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780234596744.png" title="" alt="2.png"/></p><ul style="list-style-type: disc;"><li><p>Rigorous Protocol Adherence</p></li></ul><p>Ensuring that all laboratory personnel are trained in and strictly adhere to standardized protocols can significantly reduce human error.</p><ul style="list-style-type: disc;"><li><p>Use of Barrier Methods</p></li></ul><p>Implementing physical barriers, such as gloves and face masks, and using dedicated tools for different stages of the extraction process can help prevent cross-contamination.</p><ul style="list-style-type: disc;"><li><p>Regular Equipment Maintenance and Calibration</p></li></ul><p>Keeping all laboratory equipment well-maintained and regularly calibrated minimizes the risks of mechanical errors leading to contamination.</p><ul style="list-style-type: disc;"><li><p>Quality Control Measures</p></li></ul><p>Regularly testing reagents for contamination and employing negative controls in extraction runs can help identify and mitigate contamination sources promptly.</p><ul style="list-style-type: disc;"><li><p>Employment of Enclosed Automated Systems</p></li></ul><p>Utilizing automated nucleic acid extraction systems that operate within a closed environment can drastically reduce the exposure of samples to potential contaminants.</p><p>By integrating these strategies into daily laboratory operations, researchers could lower the chances of lab contamination. It improves the reliability and accuracy of their DNA and RNA extraction results, ensuring that their findings are both valid and reproducible.</p><p><strong>Introducing Tianlong&#39;s GeneRotex 96 Nucleic Acid Extractor</strong></p><p><a href="https://www.medtl.net/products/generotex-96-nucleic-acid-extractor.html" target="_self">Tianlong&#39;s GeneRotex 96 Nucleic Acid Extractor</a>&nbsp;represents a significant advancement in the field of automated nucleic acid extraction, offering a host of features designed to minimize lab contamination. This nucleic acid extraction machine is viably engineered to handle the complexities of DNA and RNA extraction with precision and reduced risk of lab contamination. Here are the key features of this automatic nucleic acid extraction machine:</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240619/1718780254816636.png" title="" alt="3.png"/></p><ul style="list-style-type: disc;"><li><p>RMT Technology</p></li></ul><p>A distinguishing feature of the GeneRotex 96 Nucleic Acid Extractor is its use of self-developed Rotational Mixing Technology (RMT). Traditional oscillation-based mixing methods are prone to generating aerosols, which can carry nucleic acids and other contaminants, posing a substantial risk for cross-contamination. The RMT technology equipped at GeneRotex 96 Nucleic Acid Extractor reduces aerosol generation by over 50% compared to conventional techniques. By minimizing aerosol production, the risk of cross-contamination is significantly lowered, thereby enhancing the accuracy and reliability of experimental results.</p><ul style="list-style-type: disc;"><li><p>Advanced HEPA Filtration and Negative Pressure System</p></li></ul><p>The GeneRotex 96 Nucleic Acid Extractor incorporates an advanced HEPA filtration system in conjunction with a negative pressure airflow mechanism when doing DNA and RNA extraction. The built-in HEPA filter is highly efficient in capturing airborne particles, including potential contaminants. The negative pressure system ensures that expelled air is effectively filtered, greatly reducing the risk of environmental contamination.</p><p>This dual-layer protection system not only maintains the integrity of the samples but also curbs the chances of lab contamination, enhancing the safety of personnel and other experiments.</p><ul style="list-style-type: disc;"><li><p>Integrated UV Disinfection</p></li></ul><p>The GeneRotex 96 Nucleic Acid Extractor features integrated UV disinfection capabilities. Equipped with UV lamps, this automatic nucleic acid extraction system can be programmed to disinfect the working area before and after each use. UV disinfection is a well-established method for creating a sterile environment for DNA and RNA extraction, providing additional protection against contamination in the lab.</p><ul style="list-style-type: disc;"><li><p>Automated Door System</p></li></ul><p>Human error is a common source of contamination in laboratory settings. The GeneRotex 96 Nucleic Acid Extractor addresses this issue with its automated door system, which uses a motor-driven component to open and close the chamber doors without manual handling. This reduces the risk of contamination from human contact with potentially contaminated surfaces as handling the DNA and RNA extraction tasks.</p><ul style="list-style-type: disc;"><li><p>Other Features</p></li></ul><p>Except for advanced disinfect measure and automatic features to complete the DNA and RNA extraction better, here are more features of GeneRotex 96 Nucleic Acid Extractor to present the fast, high-capacity, and reliable nucleic acid result:</p><p>1.&nbsp;High Throughput and Efficiency: Capable of handling anywhere from 1 to 96 samples per run, the GeneRotex 96 Nucleic Acid Extractor accommodates 96 deep-well plates and six tube strips. This design ensures high throughput while reducing reagent waste, optimizing both resource usage and experimental scalability.</p><p>2.&nbsp;Built-in Full-Color Touch Screen:The inclusion of a 7-inch color LCD touch screen makes the system user-friendly and simplifies the operation of complex experiments.</p><p>3.&nbsp;High Purification: With less than 1% residual magnetic beads after purification, the GeneRotex 96 Nucleic Acid Extractor delivers high-purity nucleic acids, which is essential for reliable experimental results.</p><p>These innovative features make the Tianlong GeneRotex 96 Nucleic Acid Extractor an essential tool for laboratories where they seek effectiveness and efficiency of the nucleic acid extraction techniques while significantly reducing the potential for lab contamination.</p><p><strong>Conclusion</strong></p><p>Minimizing lab contamination during DNA and RNA extraction is crucial for achieving reliable and accurate molecular research outcomes. By understanding the possibility of contamination and implementing robust preventive strategies, researchers can significantly enhance the integrity of their experimental results. The adoption of advanced machines, like Tianlong&#39;s GeneRotex 96 Nucleic Acid Extractor, plays a pivotal role in this process. With features such as advanced EMT technology, HEPA Filtration, high-throughput capabilities, and more, the GeneRotex 96 Nucleic Acid Extractor sets a new standard in minimizing contamination risks.</p><p>This nucleic acid extraction machine is also an embodiment of Tianlong&#39;s extensive experience and commitment to innovation in the molecular study industry, positioning us as a trusted leader. By choosing us, Tianlong, researchers not only invest in superior technology but also gain a partner renowned for its expertise and reliability in facilitating groundbreaking molecular discoveries.&nbsp;You can visit <a href="https://www.medtl.net/" target="_self">Tianlong</a>’s official website to learn more.</p>"
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  ["title"] => string(25) "Eid Mubarak from Tianlong"
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  ["title"] => string(57) "Video | Biolum Pro Portable ATP Hygiene Monitoring System"
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  ["title"] => string(31) "Happy Dragon Boat Festival 2024"
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  ["title"] => string(131) "Tianlong Promotes Environmental Sustainability and Hygiene Monitoring with its Biolum Portable ATP Hygiene Monitoring System Series"
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  ["content"] => string(3761) "<p>In honor of World Environment Day on 5 June, Tianlong, an innovative R&amp;D company specializing in molecular diagnostic products, demonstrates its dedication to environmental sustainability and cleanliness through the introduction of the Biolum Portable ATP Hygiene Monitoring System Series.&nbsp;Utilizing advanced technology, Tianlong is transforming the way hygiene standards are monitored and maintained in various environments.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240605/1717566645826074.png" title="" alt="1.png" width="687" height="521" style="width: 687px; height: auto;"/></p><p>Tianlong&#39;s Biolum Portable ATP Hygiene Monitoring System, a powerful&nbsp;tool for implementing and overseeing hygiene monitoring programs, has achieved outstanding performance, earning certification from the prestigious AOAC Research Institute&#39;s Performance Tested Methods (PTM) Program. The PTM Program developed more than 30 years ago provides independent third-party review and certification for proprietary test method performance, and has played a crucial role with emerging health concerns by providing a widely accepted certification in a fast-to-market context.</p><p>&nbsp;</p><p>This certification offers users a reliable assessment of the product&#39;s performance, ensuring it meets the highest standards for its intended purpose, and protecting users through trusted, reliable testing methods and products. This validation underscores Tianlong&#39;s commitment to excellence and quality, setting a benchmark for reliability and effectiveness in hygiene monitoring systems.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240605/1717566710135174.png" title="" alt="微信截图_20240605135136.png"/></p><p>Through relentless dedication to innovation, Tianlong has introduced the Biolum Pro, the next-generation hygiene monitoring system. With the ability to generate results in just 10 seconds and a remarkable detection sensitivity of down to 10<sup>-16</sup>&nbsp;mol of ATP, the Biolum Pro sets a new standard in efficiency and accuracy.&nbsp;The intuitive smartphone design with 5-inch touch shatter-proof screen ensures user-friendly operation. Cloud-based data management enables users to monitor, track, and trend testing results across multiple facilities, making risk management easier than ever.&nbsp;The&nbsp;Biolum Pro innovates to make everything easier and maximize its value to the&nbsp;business.</p><p>&nbsp;</p><p>In Russia, Tianlong&#39;s Biolum series has garnered extensive utilization in the food service sectors. Customers spanning the dairy, meat, fish, and confectionery industries have embraced the Biolum series for conducting rigorous testing to monitor cleanliness standards during production and transportation processes, a critical aspect of ensuring product quality and safety. The exceptional performance of Tianlong&#39;s Biolum series, characterized by its user-friendly interface, flexibility in application, and remarkable accuracy has been instrumental in helping the industry maintain high standards of cleanliness and hygiene. The consistent delivery of reliable results has not only bolstered customer’s confidence in the product but has also enhanced operational efficiency and quality control measures in the industry.</p><p>&nbsp;</p><p>The Biolum series sets a new standard for cleanliness assessment, ensuring not only efficiency but also a sustainable approach to environmental care.&nbsp;By integrating technological innovation with a commitment to cleanliness and sustainability, Tianlong is paving the way for a healthier&nbsp;and greener future for all.</p>"
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  ["title"] => string(31) "World Digestive Health Day 2024"
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  ["title"] => string(75) "Tianlong Participated in METROLOGIA LAB SUMMIT and SBBQ 53rd Annual Meeting"
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  ["title"] => string(77) "Portable PCR Machine: How Does It Empower Veterinarians to Test Animal Virus?"
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  ["content"] => string(11367) "<p>Veterinarians are constantly vigilant about the threat of animal viruses. The fear is twofold: first, the direct impact on animal health, where viruses can swiftly spread through herds or flocks, causes illness and death. Second, the possibility of these viruses crossing species barriers and infecting humans leads to potential public health crises.</p><p>Thus, rapid identification and containment are paramount in preventing widespread outbreaks. One efficient tool for modern veterinarians is portable Polymerase Chain Reaction machines or <a href="https://www.medtl.net/products/instruments/real-time-pcr-systems" target="_self">portable PCR machines</a>. These devices have transformed the landscape of disease detection and control by allowing veterinarians to identify animal viruses rapidly and accurately in the field.</p><p><br/></p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240513/1715569569728144.png" title="" alt="微信截图_20240513110541.png"/></p><p style="text-align: center;"><span style="font-size: 12px;"><em>(Copyright photo from https://www.freepik.com/free-photo/medium-shot-doctor-checking-dog_22881474.htm#fromView=search&amp;page=1&amp;position=10&amp;uuid=22b74e92-0544-4557-b1c4-6a055b3dd0e3)</em></span></p><p><br/></p><p><strong>Common Animal Viruses</strong></p><p>In recent years, several common animal viruses have spread globally, impacting different species:</p><p><br/></p><p>Ø&nbsp;Virus in Dogs</p><p>There has been a notable outbreak of a canine respiratory disease, which is part of the canine infectious respiratory disease complex (CIRDC). This syndrome can be caused by various bacterial and viral pathogens and is highly contagious. This animal virus has been reported in at least 10 states in the U.S., with cases estimated in the thousands. The clinical signs are often mild but can progress to severe disease and death. This has led to challenges in developing a definitive test and in implementing targeted treatment.</p><p>Ø&nbsp;Virus in Swine</p><p>Recent global surveillance has highlighted the prevalence of several viruses in the swine population, with significant implications for animal health and potential zoonotic transmission. The African Swine Fever Virus (ASFV) continues to be a major concern, with ongoing research into gene-deleted live-attenuated vaccines showing promise for protecting pigs against this highly lethal disease. Additionally, other viruses such as Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Epidemic Diarrhea Virus (PEDV) are subjects of intensive study due to their economic impact on the swine industry and their potential to cause severe disease in pigs. The genetic diversity and evolution of these animal viruses are critical areas of focus to enhance the understanding and develop effective strategies for disease management and prevention.</p><p>Ø&nbsp;Virus in Birds</p><p>While the search results do not provide specific recent outbreaks in birds, avian influenza or bird flu, particularly the H5N1 and H7N9 subtypes, has been a recurring concern worldwide. These viruses can not only cause severe disease in birds but also pose a zoonotic risk, infecting humans with serious health implications. Additional viruses of concern in birds include infectious bronchitis virus (IBV) in chickens, which leads to significant economic losses in the poultry business due to reduced egg production and growth inhibition in chickens.</p><p><br/></p><p><strong>Solutions to Detect Animal Virus</strong></p><p>For veterinarians, the choice of diagnostic testing method is critical in managing animal health and disease outbreaks. Here&#39;s a closer look at the pros and cons of each method from a veterinary perspective:</p><p>1.&nbsp;Viral Culture</p><p>It is the traditional method for confirming the presence of a live virus, which is beneficial for animal virus research and vaccine development, including its growth requirements and susceptibility to antiviral drugs.</p><p>However, viral culture is a time-consuming process, often taking days to weeks, which can delay diagnosis and treatment. Plus, it requires specialized laboratory facilities and trained personnel. And there is a risk of exposure to the pathogen for laboratory staff, which may not be available in all veterinary clinics.</p><p>2.&nbsp;Anti-Body Testing</p><p>Antibody tests are valuable for determining past exposure to a virus within a population, offering insights into the immune status and seroepidemiology. These tests are generally less expensive and provide quicker results than other diagnostic methods, making them a cost-effective option for large-scale surveys.</p><p>But this solution cannot confirm active infections and may not distinguish between different strains of a virus. Additionally, the presence of antibodies does not always equate to protective immunity, which can complicate the interpretation of results.</p><p>3.&nbsp;Antigen Testing</p><p>Antigen testing detects the presence of viral proteins, which can indicate an active infection. This method offers a relatively rapid means of diagnosis compared to viral culture and can be adapted for point-of-care testing, which is beneficial for quick decision-making.</p><p>However, antigen tests are often less sensitive than nucleic acid tests, leading to a higher risk of false negatives, especially when the viral load is low, as in the early or late stages of infection.</p><p>4.&nbsp;Nucleic Acid Testing</p><p>Although the cost of nucleic acid testing (NAT) would be higher than other solutions, NAT is highly sensitive and specific, making it the gold standard for detecting viral nucleic acids.</p><p>NAT is a type of diagnostic test that detects the genetic material of viruses or other pathogens, such as DNA or RNA. It can identify the animal virus at low levels, even before the onset of clinical symptoms, which is crucial for early detection and intervention. Besides, the NAT allows for the differentiation of virus strains and the monitoring of genetic changes, providing valuable information for tracking outbreaks and understanding transmission dynamics.</p><p style="text-align: center;"><img class="new_ueditor_box_img" src="/Public/Uploads/ueditor/upload/image/20240513/1715569701418345.png" title="" alt="微信截图_20240513110718.png"/></p><p><br/></p><p><strong>Introduction of Portable PCR Machine</strong></p><p>The Polymerase Chain Reaction (PCR) machine is a critical tool used to perform NAT. For veterinarians, the PCR machine is one of the effective tools to have PCR testing for dogs, swine, domestic fowl, or other animals. It can effectively detect the animal virus to prevent and control the spread of animal viruses and deliver the clinical solution. Among various kinds of PCR machines, the portable one is suitable for veterinarians to detect animal viruses. Talking specifically, here’s why:</p><p>Timely Intervention</p><p>Early detection of a viral outbreak is critical in controlling its spread. Portable PCR machines allow for faster identification of pathogens, which can lead to quicker interventions, reducing the impact on animal health and the potential for economic losses.</p><p>Sample Integrity Assurance</p><p>A portable PCR machine can ensure sample integrity for veterinarians. Samples analyzed immediately after collection are less likely to be compromised by degradation, ensuring that the genetic material remains intact and the results are as accurate as possible. Besides, the elimination of transport delays accelerates the diagnostic process, allowing for faster identification of diseases and immediate implementation of control measures, which is essential in managing outbreaks and preventing further spread.</p><p>Accessibility in Remote Locations<br/></p><p>Traditional PCR equipment is often large and requires a stable power supply and temperature-controlled environments, which may not be available in remote or rural areas. A portable PCR machine, with its lightweight and compact design, can be easily carried and operated in these challenging environments, empowering veterinarians with diagnostic capabilities where they are needed most.</p><p>Reasons to Choose Tianlong’s Gentier Mini+ 4 Channels Portable Real Time PCR System</p><p>Veterinarians, entrusted with the health and welfare of animal patients, must meticulously select diagnostic tools that embody precision, efficiency, and versatility. Our <a href="https://www.medtl.net/products/gentier-mini-4-channels-portable-real-time-pcr-system.html" target="_self">Tianlong&#39;s Gentier Mini+ 4 Channels Portable Real-Time PCR System</a>&nbsp;is a reliable portable PCR machine that is tailored to meet the demands of veterinary diagnostics:</p><p>Optimized Efficiency</p><p>Time is precious in veterinary practice. Otherwise, it would render animal virus outbreaks and a sum of economic loss. The Gentier Mini+ 4 Channels Portable Real-Time PCR System&#39;s remarkable capability to complete all 16 wells of fluorescence scanning within a mere second is a testament to its unrivaled efficiency. Through the integration of dual fluorescence channels, our portable PCR machine streamlines workflow processes, enabling veterinarians to expedite sample analysis without compromising accuracy.</p><p>Robust Analytical Capabilities</p><p>The Gentier Mini+ 4 Channels Portable Real-Time PCR System is a comprehensive and professional analytical tool for the detection of pathogens. Equipped with sophisticated software, our digital PCR system can empower veterinarians with a diverse array of analytical functions, ranging from qualitative to quantitative analyses. Whether discerning subtle genetic variations through SNP analysis or quantifying target sequences with absolute precision, this PCR machine affords veterinarians unparalleled insights into the molecular landscape of their patients.</p><p>Practical Portability</p><p>Adaptability means efficiency when the animal virus outbreaks or becomes an outbreak. Our Gentier Mini+ 4 Channels Portable Real-Time PCR System&#39;s compact dimensions (205mm(L)*156mm(W)*153mm(H)) and featherweight construction (3.2kg) epitomize practicality, conserving valuable bench space while facilitating seamless mobility. Its portability empowers veterinarians to conduct on-site testing in mobile laboratories with effortless ease. Moreover, the system&#39;s innate stability obviates the need for fluorescence recalibration post-relocation, ensuring uninterrupted diagnostic precision regardless of setting.</p><p><br/></p><p><strong>Conclusion</strong></p><p><br/></p><p>For veterinarians, resorting to NAT is one of the effective solutions to prevent and control the animal virus outbreak. Aims to respond fast and find out the pathogeny, our Tianlong’s Gentier Mini+ 4 Channels Portable Real-Time PCR System is an efficient tool for its compactness, fast real-time testing, and analytics ability. And we, <a href="https://www.medtl.net/" target="_self">Tianlong</a>, are a professional manufacturer of nucleic acid testing instruments and reagents with over 20+ experience. You can browse our website to learn more information about our digital PCR machines and <a href="https://www.medtl.net/contact" target="_self">contact us</a>&nbsp;easily.&nbsp;</p>"
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